Kem Izzet made his return from injury as Colchester United under-21s were beaten 4-3 at neighbours Ipswich Town.
Izzet made his first competitive appearance in nearly two months following an ankle injury when he featured in the U's game.
Alex Gilbey, Jordan Sanderson and Jack Compton were all on target for Colchester.
But Town ran out winners thanks to a hat-trick from Frank Nouble and a further strike from Andy Drury.
The likes of Matt Heath and Drey Wright also featured for the U's in the Professional Development League Two match.